Dr. Cheng's Laboratory

醱酵技術: medium evaluation, flask cultivation, bioreactor operation and design, modeling of cultivation kinetics, submerged/solid state fermentation (bacteria, yeast, fungi), aseptic techniques.

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生物材料鑑定: FTIR, X-ray diffraction analysis, viscosity meter, microscopy (FE-SEM, TEM), thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA).

 

分子生物技術: polymerase chain reaction (PCR), yeast two-hybrid, gene transformation.

 

生化分析: enzyme purification and analysis, enzyme-linked immunosorbent assay (ELISA), Western blot, Northern blot, Southern blot, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE).

 

食品分析與處理: heat exchanger, steam oven, freeze dryer, extruder, Good manufacturing practices (GMP), Sanitary standard operating procedures (SSOP), Hazard analysis and critical control points (HACCP), element analysis.

 

動物細胞與組織培養: MTT assay, flow cytometry, gene transfection, cell fusion, cell stain.

 

植物細胞與組織培養: genome DNA isolation, gene transformation, plant tissue propagation, embryo rescue.

 

蛋白質體學: mass spectrometry (GC/MALDI/SELDI; GC/MS), peptide sequencing.

 

化學分析: pH meter, chromatography (IC, HPLC, FPLC, TLC), GC-FID.

 

模擬與分析軟體: DNAsist, MATLAB, AutoCad, STELLA, finite element (FEM and BEM) computer program, MiniTab.